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Nutrient Broth

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NUB20500

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A general purpose medium for the cultivation of non-fastidious microorganisms. Dehydrated media Code number: 500 g: NUB20500, 5 kg: NUB25000 Colour: Yellowish Appearance: Homogeneous hygroscopic powder pH before autoclaving (25 °C): 7,2 – 7,6 Direction: Suspend 16 g in one litre of distilled water and heat gently to dissolve the medium completely. Dispense into final containers and sterilise by autoclaving at 121 °C for 15 minutes. Prepared media Bottled media: 100 ml: NUB30100, 500 ml: NUB30500 Tubed media: 150 x 15 mm: NUB40010 (10 ml) Colour: Yellowish pH (25 °C): 7,3 – 7,5 Direction: Dispense the bottled media aseptically into sterile final containers. Media in tubes are ready to use. FORMULA in g/l Peptones 11 Sodium chloride 5 Note: The typical formula can be adjusted to obtain optimal performance. Storage conditions: Store the dehydrated media tightly closed in a dry place at room temperature. Store the bottled and tubed media protected from light at room temperature. Use before the expiry date on the label. Quality control: Test strains Incubation temp: 37 °C Growth Incubation time: 24 h Escherichia coli ATCC 25922 Good Staphylococcus aureus ATCC 29213 Good References: APHA (1985) Standard Methods for the Examination of Water and Wastewater In vitro diagnostic – for professional use only! 

NUB20500
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